Ion Transport: Ion Carriers

CARRIERS THAT UTILISE ATP (PUMPS)
  • Na/K ATPase
    • Alpha and beta subunit
    • Alpha
      • Coordinate cation transport
        • 3Na efflux, 2K influx
        • Maintain Na K concentration gradient
        • In epithelial cells
          • Drive Na and K transport
        • In non-epithelial cells
          • Determine resting membrane potential
          • Allow depolarisation
          • Allow electrical signalling
      • Bind and hydrolyse ATP to release energy to drive transport
    • Drugs targeting Na/K ATPase
      • Digoxin
        • Increasing cardiac contractility
          • Inhibits ATPase enzyme of Na/K ATPase
          • Preventing hydrolysis
          • Removes energy supply
          • Inhibit Na efflux, K influx
          • Rise in intracellular Na
          • Rise in intracellular Ca
          • Enhancing actin-myosin interaction
          • Increasing cardiac contractility
          • ?Significant inotropic in heart failure treatment
        • Enhances vagal activity
          • Slow conduction via atrioventricular node
          • Used in rate control in atrial fibrillation

CARRIERS THAT UTILISE SECONDARY ACTIVE TRANSPORT MECHANISMS
  • Energy from electrochemical gradient of one ion
  • Na/K/2Cl cotransporter
    • Mediates Na transport across epithelia
    • Thick ascending limb of the loop of Henle
    • Reabsorption 25% Na
    • Drugs targeting Na/K/2Cl co transporter
      • Loop diuretics (frusemide)
        • Causes natriuresis
        • Block Na/K/2 Cl cotransporter
        • Reducing Na and K reabsorption
    • Disease caused by abnormal Na/K/2Cl co transporter
      • Bartter's syndrome
        • Rare
        • Profound hypokalaemia in infancy
  • Na/Cl co-transporter
    • Mediates Na transport across epithelia
    • Distal tubule
    • Reabsorption of 10% Na
    • Drugs targeting Na/Cl co-transporters
      • Thiazide diuretics
        • Causes natriuresis
        • Blocks Na/Cl co-transporter
        • Reducing Na and Cl reabsorption
    • Disease caused by abnormal Na/Cl co-transporters
      • Gitelman's syndrome
        • Hypokalaemia in adults

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